Protein elicitors HSyp1 and HSyp2 from Saccharothrix yanglingensis Hhs.015 enhance disease resistance of plants
[Objective]Preliminary studies have identified four protein elicitors from the whole genome of Saccharothrix yanglingensis Hhs.015 that can induce a hypersensitive response(HR)in Nicotiana benthamiana.This study investigated the two elicitors capable of triggering a strong HR and their associated immune mechanisms,aiming to provide a basis for probing into the molecular mechanisms by which Hhs.015 enhances the disease resistance of plants.[Methods]The protein elicitors capable of inducing the immune responses of plants were screened based on the HR and reactive oxygen species(ROS)levels.A prokaryotic expression system was used to purify the proteins.Furthermore,we verified the roles of the protein elicitors in inducing plant disease resistance by observing the disease spots,measuring the activities of defense-related enzymes,and analyzing the expression of defense genes.Additionally,we treated Arabidopsis thaliana seedlings with the protein elicitors to evaluate the plant growth-promoting effects of the elicitors.[Results]The protein elicitors HSyp1 and HSyp2 from Hhs.015 that could induce a HR,callose and ROS deposition,and upregulation of defense genes in N.benthamiana,were screened and found to be soluble.HSyp1 and HSyp2 enhanced the resistance of different plants to Sclerotinia sclerotiorum,Phytophthora capsica,and Valsa mali.Furthermore,HSyp1 and HSyp2 promoted the cotyledon and root development of A.thaliana seedlings,and HSyp2 exhibited stronger plant growth-promoting effect than HSyp1.[Conclusion]This study identified two protein elicitors HSyp1 and HSyp2 capable of triggering immune responses,enhance disease resistance,and promoting growth of plants.The findings provide a theoretical basis for the development of new biocontrol agents and offer experimental evidence for their future field application.
prokaryotic expressionprotein elicitorshypersensitive responsedisease resistance of plantsplant growth-promoting effect
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维普
