Correlation analyses of substances and pathways of Tuber sinenses and their symbiotic pine roots
Truffles must be symbiotic with plants to form ectomycorrhiza(ECM),which facilitates the formation of fruiting bodies by mutually beneficial exchanges of substances.[Objective]To elucidate the flow of substances between Tuber sinense and Pinus yunnanensis in a symbiosis relationship.[Methods]Liquid chromatography-mass spectrometry(LC-MS)was employed to analyze the metabolite profiles of freshly harvested T.sinense(SL)and P.yunnanensis roots(SG).[Results]In SL and SG,1 304 and 1 516 substances were detected respectively,including 399 SL-specific substances(SLSs)and 611 SG-specific substances(SGSs).There were 294 common differential substances,including 93 up-regulated substances(DEMs-up)and 201 down-regulated substances in SL.The network correlation analysis revealed that 92 of the 100 substances with the relative content ≥2.0(40 SLSs and SGSs and 60 DEMs-up)displayed significant correlations.Twenty-nine associated substances in SLSs and SGSs and 19 substances in DEMs-up were enriched in 15 important pathways,which mainly involved secondary metabolite biosynthesis,amino acid biosynthesis and metabolism,and vitamin biosynthesis and metabolism.[Conclusion]A network correlation existed between T.sinense and P.yunnanensis pine roots metabolites.Some substances in truffles and pine roots,including adenosine 3',5'-cyclic monophosphate(cAMP),phytosterol,and indole-3-acetic acid,had regulatory effects on the development of fruiting bodies.The findings provided a theoretical basis for further studying the potential signaling molecules associated with fruiting body development and elucidating the symbiotic mechanism between T.sinense and pines.Additionally,this study laid a practical basis for screening the substances promoting the growth of mycorrhizal seedlings and fruiting bodies and for large-scale artificial cultivation of truffles.
万方数据
维普
