Truffles must be symbiotic with plants to form ectomycorrhiza(ECM),which facilitates the formation of fruiting bodies by mutually beneficial exchanges of substances.[Objective]To elucidate the flow of substances between Tuber sinense and Pinus yunnanensis in a symbiosis relationship.[Methods]Liquid chromatography-mass spectrometry(LC-MS)was employed to analyze the metabolite profiles of freshly harvested T.sinense(SL)and P.yunnanensis roots(SG).[Results]In SL and SG,1 304 and 1 516 substances were detected respectively,including 399 SL-specific substances(SLSs)and 611 SG-specific substances(SGSs).There were 294 common differential substances,including 93 up-regulated substances(DEMs-up)and 201 down-regulated substances in SL.The network correlation analysis revealed that 92 of the 100 substances with the relative content ≥2.0(40 SLSs and SGSs and 60 DEMs-up)displayed significant correlations.Twenty-nine associated substances in SLSs and SGSs and 19 substances in DEMs-up were enriched in 15 important pathways,which mainly involved secondary metabolite biosynthesis,amino acid biosynthesis and metabolism,and vitamin biosynthesis and metabolism.[Conclusion]A network correlation existed between T.sinense and P.yunnanensis pine roots metabolites.Some substances in truffles and pine roots,including adenosine 3',5'-cyclic monophosphate(cAMP),phytosterol,and indole-3-acetic acid,had regulatory effects on the development of fruiting bodies.The findings provided a theoretical basis for further studying the potential signaling molecules associated with fruiting body development and elucidating the symbiotic mechanism between T.sinense and pines.Additionally,this study laid a practical basis for screening the substances promoting the growth of mycorrhizal seedlings and fruiting bodies and for large-scale artificial cultivation of truffles.
维普
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