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细菌解磷能力测定方法的研究

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选择分解有机磷能力较强的3株细菌和溶解磷矿粉较强的4株细菌,砂培4周后,用不同的方法测定水浸提液中磷的含量。发现不同的细菌解磷能力差异很大,细菌在分解磷化合物的同时,一部分磷被细菌同化,一部分以无机磷酸盐状态贮藏在细菌细胞内。直接测定浸提液中无机磷酸盐的含量,将大大低估细菌的解磷能力,必须将浸提液消煮,才能比较正确地反映细菌分解磷的能力。
THE METHODS FOR QUANTIFYING CAPACITY OF BACTERIA IN DISSOLVING P COMPOUNDS
Three bacteria of decomposing lecithin and 4 bacteria of dissolving aptite were incubated for 4 weeks with sand media respectively. Phosphorus in the sand was extracted with distilled water and measured by different methods. It was found that the bacteria have a quite different ability to release P from the materials. Part of the P released became organic phosphorus compounds in microbial tissue. However, a large amount of the P was reserved in microbial cells in a form of phosphates. The direct measurement of P in the extract by molybdenum blue method would underestimate the capacity of the bacteria to release P from the materials. The correct approach was that the sand was fumigated with chloroform and then digested with acid before the measurement by molybdenum blue method.

赵小蓉、林启美、孙焱鑫、姚军、张有山

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中国农业大学土壤和水科学系

北京市农林科学院植物营养与资源研究所

解磷细菌 熏蒸 消煮

北京市自然科学基金

6971003

2001

微生物学通报
中科院微生物所 中国微生物学会

微生物学通报

CSCD北大核心
影响因子:1.052
ISSN:0253-2654
年,卷(期):2001.(1)
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