Advances in the identification of Bacillus subtilis and closely related species
Bacillus subtilis and closely related species,as members of the "subtilis-group",are composed of rod-shaped,endospore-forming bacteria with high degree of phenotypic and biochemical similarity.The "subtilis-group" includes B.subtilis,B.licheniformis,B.pumilus,B.amyloliquefaciens,B.atrophaeus,B.mojavensis,B.vallismortis,B.sonorensis,B.tequilensis,B.siamensis.Strains of the group have been exploited for biotechnological,industrial and agricultural applications; therefore,their rapid and accurate identification is important in practice.Here we reviewed traditional and molecular methods to identify these species.The combination of the physiological and biochemical characteristics,sequencing analysis and species-specific PCR would allow us to rapidly and accurately differentiate the strains of the group.