Culture-independent analysis of intestinal microbial diversity from Rhinopithecus roxellana
[Objective] The composition and diversity of intestinal microbial diversity of wild Sichuan snub-nosed monkey (Rhinopithecus roxellana) were investigated.[Methods] The total DNA extracted from endophytic bacterial was performed 16S rRNA gene specific amplification by used bacteria-specific primers 799F and 1492R and constructed a clone library.Then,16S rRNA gene phylogenetic tree was established by analyzed positive clones used polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and by sequenced some of the clones.[Results] The 157 clones analyzed were grouped into 27 operational taxonomic units (OTUs),where the clone distribution was as follows:62.10％ of Firmicutes which contained Clostridium,Cellulosilyticum,Robinsoniella,Anaerofustis,Anaerovorax and Blautia,37.90％ of uncultured bacteria.[Conclusion] The intestine bacterial of wild Sichuan snub-nosed monkey has a rich diversity and maybe exist new taxon.
NETL
NSTL
万方数据
维普
