Isolation, identification and phosphate solubilization analysis of phosphate-solubilizing bacteria derived from banana rhizosphere soil
[Objective] In this study,ground phosphaterock was used as the undissolved phosphorus to select the high-efficient phosphate-solubilizing bacteria from the banana rhizosphere soil.[Methods] The methods of halo ring and Mo-Sb colorimetry were applied to isolate the phosphate-solubilizing bacteria.And a combination of morphologic alcharacteristics identification,physiological and biochemical test,16S rRNA gene sequences analysis and phylogenic trees were employed to determine the genus of the phosphate-solubilizing bacteria.Various sources of carbon or nitrogen,the C/N value (40∶1,20∶1,8∶1) and the pH value of culture medium which may affect the bacteria's ability to dissolve the ground phosphate rock were studied with the single factor experimental method.[Results] Eight strains of phosphate-solubilizing bacteria (PSB) were isolated from the root soil,and three of them,B3-5-6,M-3-01,T1-4-01,were taken as representatives.The B3-5-6 was identified to be Bacillus aerophilus,M-3-01 Serratia nematodiphila and T1-4-01 Enterobacter asburiae.The phosphate-solubilizing ability of B3-5-6 and the pH of the culture medium was found to be linear negative correlated (|r|=0.949 66＞0.735) and reached the extremely significant level.For strain B3-5-6,the phosphate-solubilizing ability was better when the carbon source was sucrose,nitrogen source was (NH4)2SO4 and C/N was 40∶1.For strain T1-4-01,the phosphate-solubilizing ability was better when the carbon source was glucose,nitrogen source was (NH4)2SO4 and C/N was 20∶1.For strain M-3-01,the phosphate-solubilizing ability was better when the carbon source was glucose,nitrogen source was (NH4)2SO4 and C/N was 20∶1 for strain T1-4-01,the phosphate-solubilizing ability was better when the carbon source was lactose,nitrogen source was peptone and C/N was 20∶1.The phosphate-solubilizing ability was raised 1.12 fold,1.17 fold and 2.55 fold,respectively.[Conclusion] Various sources of carbon or nitrogen,and C/N could affect the phosphate-solubilizing ability of different strains.The phosphate-solubilizing ability of B3-5-6 and the pH of the culture medium was found to be linear negative correlated (|r| =0.949 66＞0.735) and reached the extremely significant level.The phosphate-solubilizing mechanism of this strain needs to be furtherly investigated.
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维普
