Isolation, screening and preliminary identification of endophytic actinobacteria from Ginkgo biloba
[Objective] To isolate and screen actinomycetes with antimicrobial activity from Ginkgo biloba,and provide new resources for the application of actinomycetes in biological control.[Methods] The cultural method of plant tissue paste on medium were used to isolate actinomycetes.[Results] In total,we have isolated 156 endophytic actinomycetes from Ginkgo biloba.The fermentation broth of 47 strains could inhibit growth of the tested phant pathogenic fungi,and the strain KLBMP 5501 exhibited extensive antagonism on most of tested pathogens.Based on the morphological characteristics,cultural characteristics,physiological characteristics and 16S rRNA gene sequence similarity analysis,strain KLBMP 5501 was identified as Streptomyces violascens,a known species of Streptomyces.[Conclusion] Actinomycetes strains with high antimicrobial activity were obtained and showed potential for biological control of plant diseases.One of these strains,KLBMP 5501,was identified.
NETL
NSTL
万方数据
维普
