Diversity of culturable bacteria isolated from Periplaneta americana adult gut
[Objective] To understand the diversity of culturable bacteria isolated from Periplaneta americana adult gut.[Methods] Bacteria were isolated from the sample by using conventional culture-dependent method and then investigated by using numerical taxonomy and phylogenetic analysis based on 16S rRNA gene sequences.[Results] Totally 54 bacteria strains were selected on nutrient agar plate.On the basis of morphological,physiological and biochemical characteristics,we selected 32 strains to perform a phylogenetic analysis based on 16S rRNA gene sequences.The results showed that the strains can be divided into 12 apparent groups on the 82％ similarity level by numerical taxonomy; 32 isolates represented 20 species,belonging to 15 genera of 10 families in four phylogenetic groups (Proteobacteria,Bacteroidetes,Firmicutes,and Actinobacteria).The most abundant and diverse isolates were within the Proteobacteria (15 strains,46.9％) and the Bacteroidetes (10 strains,31.3％).[Conclusion] Overall,the study results presented above demonstrated that there are abundant bacteria diversity in the Periplaneta americana adult gut.
Periplaneta americana adultCulturable bacteriaPhylogenetic analysisBacterial diversity
NETL
NSTL
万方数据
维普
