Composite organic acids for decontamination of Listeria monocytogenes in chicken
[Background]Listeria monocytogenes(Lm)is a major zoonotic pathogen.Lm carried by domesticated animals can spread along slaughter and sale chains,posing a threat to consumers'health.[Objective]To establish and apply a green efficient technology for the control of Lm contamination in the broiler industrial chain.[Methods]We prepared a composite organic acid disinfectant composed of lactic acid and citric acid,optimized the sterilization conditions by response surface methodology,conducted preliminary applications of the disinfectant in slaughterhouses,and explored the sterilization mechanism.[Results]The optimal sterilization conditions were as follows:lactic acid content of 0.98%,citric acid content of 0.72%,and treatment for 3.6 min.The treatment under these conditions reduced the Lm on the chicken surface by more than 99%,extended the shelf life of chicken,and decreased the isolation rate of Lm on the surface of knives and broiler carcasses in the pre-cooling and segmentation stages in broiler slaughterhouses.Moreover,the composite organic acid disinfect significantly down-regulated the expression of genes associated with Lm virulence,inhibited the biofilm formation,and destroyed the cell structure of Lm.[Conclusion]The composite organic acid disinfect prepared in this study can effectively reduce Lm on the broiler surface in a short time,demonstrating good application prospects in preventing and controlling Lm contamination in the slaughter process of broilers.
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