Bacteria from porcine colon regulate cytokine and transcription factor expression in the gut of the mouse model of colitis
[Background]Microorganisms in the mammalian digestive tract are important for maintaining gut homeostasis,while the regulatory role of gut bacteria in cytokine expression remains unclarified.[Objective]To compare the regulatory effects of different isolated from porcine intestinal tract on the expression of cytokines and transcription factors in the model of colitis.[Methods]Bacteria were isolated from the porcine colon,and the culture supernatant of each isolate was used to treat the Caco-2 cell model of lipopolysaccharide-induced inflammation.Furthermore,a mouse model of colitis was induced by dextran sodium sulfate(DSS)and then administrated with bacterial suspension by gavage.The gene expression of cytokines and transcription factors in Caco-2 cells and mouse colon tissue,the cell growth rate,and the mouse weight after bacterial treatment were determined.[Results]Six strains of bacteria were isolated from porcine colon,belonging to Enterococcus,Lactobacillus,Sharpea,and Mitsuokella.In the Caco-2 cell model of inflammation,Lactobacillus amylovorus LGM down-regulated the mRNA levels of interleukin(IL)-4 and IL-17(P<0.05);Enterococcus cecorum LGM down-regulated the mRNA levels of T-box expressed in T cells(T-bet),forkhead box P3(Foxp3),IL-17,and transforming growth factor-β(TGF-β)(P<0.05).In the mouse model of colitis,only L.amylovorus LGM alleviated the colon inflammation,restored the colon length,and up-regulated the mRNA levels of Foxp3,GATA Binding Protein 3(GATA-3),and interferon-γ(IFN-γ)in the colon tissue(P<0.05).However,Mitsuokella jalaludinii LGM aggravated colon injury and up-regulated the mRNA level of interferon-γ in the tissue(P<0.05).[Conclusion]The bacterial isolates have different regulatory effects on porcine colitis,which is related to the regulation of cytokine gene expression.L.amylovorus LGM exerts an anti-inflammatory effect by up-regulating the expression of Foxp3 and GATA-3 in the colon.
gut microorganismspigslactic acid bacteriacolitiscytokines
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