[背景]组蛋白乙酰转移酶general control nonderepressible-5(GCN5)作为一种重要的表观遗传修饰因子,参与调控真核生物的多种生命活动.目前,关于灰葡萄孢(Botrytis cinerea)组蛋白乙酰转移酶GCN5的功能与机制研究尚未见报道.[目的]明确灰葡萄孢组蛋白乙酰转移酶GCN5的编码基因BcGCN5在病菌生长发育和致病过程中的功能,为进一步阐明组蛋白乙酰化修饰在病原菌生长发育及致病过程中的功能与调控机制奠定基础.[方法]利用基因敲除技术构建了灰葡萄孢BcGCN5基因的敲除突变体△BcGCN5;以野生型菌株B05.10为对照,对突变体△BcGCN5的表型和致病力进行分析.[结果]灰葡萄孢BcGCN5基因的敲除突变体△BcGCN5的生长速率明显减慢,菌核数量较少,分生孢子数量显著降低,对番茄果实和烟草叶片的致病力减弱,产酸能力降低.[结论]灰葡萄孢组蛋白乙酰转移酶BcGCN5正调控病菌的生长、发育和致病过程.
Function of histone acetyltransferase BcGCN5 in Botrytis cinerea
[Background]The histone acetyltransferase general control nonderepressible-5(GCN5)is a key epigenetic modifier that plays a role in regulating biological processes in eukaryotes.At present,little is known about the function and mechanism of GCN5 in Botrytis cinerea.[Objective]To analyze the function of BcGCN5 and lay a foundation for elucidating the function and regulatory mechanism of histone acetylation in the growth,development,and pathogenicity of B.cinerea.[Methods]The mutant △BcGCN5 of B.cinerea was successfully constructed by gene knockout.The phenotype and pathogenicity of △BcGCN5 were analyzed with the wild-type strain B05.10 as the control.[Results]△BcGCN5 showed slow growth,reduced production of sclerotia and conidia,and diminished acid production,which ultimately led to weakened pathogenicity towards tomato fruits and tobacco leaves.[Conclusion]BcGCN5 positively regulated the growth,development,and pathogenicity of B.cinerea.
Botrytis cinereahistone acetyltransferasegrowth and developmentpathogenicity
NETL
NSTL
万方数据
