Effects of Soybean Culture Media with Different Varieties on the Metabolic Components of Paecilomyces hepiali Mycelium
The effects of different varieties on soybean as culture medium on the metabolic components of Paecilomy-ces hepiali were studied,and the best variety on soybean was selected to provide scientific basis for the standardized production of P.hepiali.At first,the principal component contents of 15 different varieties on soybean media were de-termined,and the mycelium biomass,adenosine content and total adenosine content of P.hepialid cultivated in differ-ent varieties on soybean medium were determined.Then the metabolic components of P.hepiali mycelium cultivated in different varieties on soybean medium were studied by non-targeted metabonomics.The results showed that there were significant differences in the contents of crude protein and crude fat in 15 different varieties on soybean cake and the content of adenosine in the mycelium of P.hepiali cultivated in the corresponding culture medium.The adenosine content of mycelium cultured in soybean medium No.9 was the highest(1 089.35±25.51)mg/kg and that of No.5 was the lowest(60.68±9.56)mg/kg.There were significant differences among mycelium cultured in No.5,No.9 and No.10(P＜0.05).Metabonomic studies showed that the relative contents of metabolic components in mycelia of No.5,No.9 and No.10 were quite different.The relative contents of amino acids such as alanine,glutamic acid,aspartic acid,stachyose,galactose and tricarboxylic acid cycle products such as 2-oxobutyric acid and citric acid in mycelium 9 were significantly higher than those in mycelia 5 and 10.KEGG enrichment analysis showed that medium 9 increased the content of adenosine by promoting the metabolic pathway of aspartic acid and glutamate and increasing the content of aspartic acid and glutamic acid.Different varieties on soybean medium have significant effect on the adenosinecontent of P.hepiali cerevisiae mycelium,and the adenosine content of mycelium cultured in No.9 soybean medium is the highest.This study provides a scientific basis for screening the best varieties on soybean in the breeding process of P.hepiali.
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