Cloning and Analysis of Ketone Synthase Domain Gene from Marine Streptomyces
Marine Streptomyces can synthesize many polyketides(PKs)with medicinal value and diverse structures and functions by polyketide synthase(PKS).The ketone synthase domain(KS),as one of the core domain in PKS,can catalyzes the decarboxylation condensation between substrates and elongated polyketones,and plays an important role in polyketide biosynthesis.In this paper,one ks gene was cloned from Streptomyces sp.X66 genomic DNA,bioinformatic analysis showed that the gene sequence was 945 bp.Blast sequence comparison showed that it had a typ-ical functional region of the ketosynthase domain.Physio-chemical analysis showed that it was intended to encode 309 amino acids,its theoretical isoelectric point was 6.60.The atomic composition was C1401H2239N425O419S8,the instabili-ty coefficient was 42.11,with average hydrophilicity at 0.112,it encoded an acidic hydrophobic unstable protein without signal peptide and transmembrane structure,and its secondary structure was mainly random coil and α-helix.Its SDS-PAGE showed that its molecular weight was about 55 kDa,which was consistent with the expected.It will provide a scientific basis for further analysis of the regulatory mechanism in the synthesis and metabolism of polyketide compounds,and the synthesis of polyketide compounds by combinatorial biology and in vitro enzymes.
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