Research Progresses of Gene Editing and Metabolic Regulation in Saccharomyces cerevisiae Based on CRISPR/Cas9 tools
The CRISPR/Cas9 systems have been widely used for gene editing and metabolic engineering in various or-ganisms.The basic principles and practical applications of the CRISPR/Cas9 in Saccharomyces cerevisiae have been summarized in this review.Firstly,the history of CRISPR/Cas9,successful cases of gene deletion and multiple DNA fragment insertion in genome of S.cerevisiae have been reviewed.Such advanced systems have reduced labor,en-hanced the understanding of molecular genetics,and accelerated the development of microbial engineering.Further-more,the research progress of the CRISPR/Cas9-based systems in the production of high value-added chemicals and the improvement of stress tolerance in S.cerevisiae have been summarized.Therefore,this review should has an im-portant reference value for genetic and synthetic biology studies based on S.cerevisiae.
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