酿酒酵母中基于CRISPR/Cas9工具的基因编辑与代谢调控研究进展
Research Progresses of Gene Editing and Metabolic Regulation in Saccharomyces cerevisiae Based on CRISPR/Cas9 tools
安迎锋 1梁瑶崑 1高嵩 1Leonid N Valentovich2
作者信息
- 1. 沈阳农业大学生物科学技术学院,辽宁沈阳 110161
- 2. National Academy of Sciences of Belarus,Institute of Microbiology,Belarus,Minsk 220084
- 折叠
摘要
CRISPR/Cas9系统已广泛用于各种生物体的基因编辑和代谢工程.本文综述了CRISPR/Cas9在酿酒酵母中的基本原理和实际应用.首先总结了CRISPR/Cas9技术的发展历史、酿酒酵母基因组中基因缺失和多DNA片段插入的成功案例.这一先进的系统减少了劳动力,增强了对分子遗传学的理解,加速了微生物工程的发展.其次总结了基于CRISPR/Cas9的系统在生产高附加值化学品和提高酿酒酵母耐应激性方面的研究进展.该综述对酿酒酵母的遗传和合成生物学研究具有重要的参考价值.
Abstract
The CRISPR/Cas9 systems have been widely used for gene editing and metabolic engineering in various or-ganisms.The basic principles and practical applications of the CRISPR/Cas9 in Saccharomyces cerevisiae have been summarized in this review.Firstly,the history of CRISPR/Cas9,successful cases of gene deletion and multiple DNA fragment insertion in genome of S.cerevisiae have been reviewed.Such advanced systems have reduced labor,en-hanced the understanding of molecular genetics,and accelerated the development of microbial engineering.Further-more,the research progress of the CRISPR/Cas9-based systems in the production of high value-added chemicals and the improvement of stress tolerance in S.cerevisiae have been summarized.Therefore,this review should has an im-portant reference value for genetic and synthetic biology studies based on S.cerevisiae.
关键词
次生代谢产物/代谢工程/合成生物学/抗逆性/酿酒酵母Key words
secondary metabolites/metabolic engineering/synthetic biology/stress resistance/Saccharomyces cerevisiae引用本文复制引用
基金项目
国家自然科学基金(31870784)
沈阳市科技计划(22-315-6-19)
沈阳市科技计划(21-110-3-19)
辽宁省教育厅科研项目(LJKZ0660)
出版年
2024
NETL
NSTL
万方数据