施用生物炭对葡萄园土壤理化性质和细菌群落结构的影响
Effects of Biochar Application on Soil Physicochemical Properties and Bacterial Community Structure in Vineyard
郑兰香 1常洁 2王芳2
作者信息
- 1. 宁夏大学 生态环境学院,宁夏 银川 750021;中国葡萄酒产业技术研究院,宁夏 银川 750021
- 2. 宁夏大学 地理科学与规划学院,宁夏 银川 750021
- 折叠
摘要
生物炭作为土壤调理剂在农业生产和环境修复中备受关注.本研究对宁夏贺兰山东麓的葡萄园施用不同量的生物炭对其土壤理化性质和细菌群落结构的影响进行了试验.设置 0、5、10 和 15 t/hm2 四种不同生物炭施用量的田间处理,施用6 个月后测定表层0～20 cm土壤的理化性质,并通过高通量测序技术分析土壤微生物的群落组成.结果显示,施用生物炭后土壤中有机碳、全氮、全磷含量和pH均有不同程度地提高.施加生物炭可以增加土壤细菌的丰富度和多样性.不同生物炭施用量下的优势菌门为放线菌门(Actinobacteria,36.66%～37.64%)、变形菌门(Protebacteria,18.74%～22.42%)、绿弯菌门(Chloroflexi,9.72%～15.03%)、酸杆菌门(Acidobacteria,5.14%～8.94%)和厚壁菌门(Firmicutes,3.7%～6.7%).优势菌纲为放线菌纲(Actinobacteria,23.09%～28.88%)、α-变形菌纲(Alphaproteobacteria,13.41%～14.4%).土壤pH(P=0.229)和有机碳(P=0.237)是对细菌群落影响最大的环境因子.
Abstract
As a soil conditioner,biochar has attracted much attention in agricultural production and environmental remediation.To study the effect of biochar application on physicochemical properties and bacterial community struc-ture of vineyard soil,biochar application experiments in vineyard at eastern foot of Mt.Helan in Ningxia were carried out.Field treatments of four different biochar application amounts of 0,5,10 and 15 t/hm2 were set up,the physico-chemical properties of soil at 0-20 cm of the surface were determined 6 months after,and the community composition of soil bacterial was analyzed through high-throughput sequencing technology.The results showed that the application of biochar can increase soil organic carbon,total nitrogen,total phosphorus and pH.Application of biochar also in-creases the richness and diversity of soil microorganisms.The dominant phyla under different biochar application lev-els are Actinobacteria(36.66%-37.64%),Protebacteria(18.74%-22.42%)and Chloroflexi(9.72%-15.03%),Ac-idobacteria(5.14%-8.94%)and Firmicutes(3.7%-6.7%).The dominant class are Actinobacteria(23.09%-28.88%)and Alphaproteobacteria(13.41%-14.4%).pH(P=0.229)and organic carbon(P=0.237)are the two greatest environmental factors that impact on soil microbial communities.
关键词
生物炭/葡萄园/土壤理化性质/细菌群落结构/高通量测序Key words
biochar/vineyard/soil physicochemical properties/bacterial community structure/high-throughput se-quencing引用本文复制引用
基金项目
国家重点研发项目(2019YFD1002500)
宁夏科技厅重点研发项目(2019BBF02024)
出版年
2024
NETL
NSTL
万方数据