Microbial Diversity and Metabolic Function Prediction in Abandoned Mine Environment
As one of the main environmental pollutions,mine wastewater in this study,sampled from an abandoned coal mine in Zunyi City,Guizhou Province,was assayed with 16S rRNA gene sequencing technology,to reveal its bacterial and archaeal community and composition of the surface sediments along the mine galleries.The results showed that bacterial diversity and abundance in all samples were higher than that of archaea,the bacterial community consisted of 52 phyla and 820 genera.The dominant bacterial were genera level of the family of norank_f_A4b,and genera level of the order of norank_f_SBR1031,and genera level of the class of norank_f_KD4-96.Archaeal commu-nity consisted of 12 families and 58 genera,with the dominant bacterial phylum being Crenarchaeota,and dominant bacterial genera provisionally as Candidatus_Nitrocosmicus and provisionally as Candidatus_Nitrsosphaera.PICRUSt2 function prediction suggested that bacteria and archaea exhibited three sulfur metabolism cycle pathways respectively were assimilatory sulfate reduction and thiosulfate oxidation of SOX complex,and archabacteria included two sulfur metabolism pathways,respectively were assimilatory sulfate reduction thiosulfate oxidation of SOX complex,with a to-tal of 18 bacterial sulfur metabolism genes and 13 archaeal sulfur metabolism genes were determined.In this study,the analyses of microorganism and its metabolic function in abandoned mine environment can further expand the under-standing of mine environmental microbial system.
mine environmentbacterial and archaeal communitiesdiversityfunctional predictionsulfur metabo-lism cycle
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万方数据
