首页|两株高效解磷菌的筛选及解磷性能分析

两株高效解磷菌的筛选及解磷性能分析

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为获得能够把难溶性磷转化为可溶性磷的解磷微生物,从茅台酒厂种植地的土壤中分离筛选出两株具有解磷能力的菌株,并对这两株菌株进行分子生物学鉴定及解磷性能分析,测定其发酵液中的有机酸,初步探究解磷机理和在酒糟中的生长能力.结果表明,筛选的P1 和P2 菌株分别为路氏肠杆菌(Enterobacter ludwi-gii)和分散泛菌(Pantoea dispersa).P1 和 P2 单菌培养的难溶磷降解率分别在第 10 天和第 1 天达到最高9.62%和7.56%,P1 和P2 混菌(P3)培养难溶磷的降解率在第2 天达到最大为18.83%.不同条件下菌株对难溶磷的降解率有一定影响,其中 40℃混菌培养在第 6 天高达 33.82%.P1 主要产乙酸,高达 4 103.65 mg/L,P2 主要产乙酸和琥珀酸,混菌培养主要产乙酸,其次是草酸.两株菌在酒糟中具有较强的生长能力,为解磷功能菌株制备酒糟生物有机肥提供一定的参考.
Screening and Analysis of Two Strains of High Efficiency Phosphor-Soluble Bacteria
In order to obtain phosphorus soluble microorganisms that can convert insoluble phosphorus into soluble phosphorus,two strains with phosphorus soluble ability were isolated and screened from soil of planting field of Maotai Distillery,and molecular biological identification and phosphorus soluble performance of both strains analysis were carried out,and organic acids in their fermentation broth were determined,and the phosphorus soluble mechanism and growth ability in distillers′grains were initially explored.The results showed that the selected strain P1 and strain P2 were Enterobacter ludwigii and Panthenia dispersus respectively.Regarding the degradation rate of insoluble phosphor-us,the highest was at 9.62% and 7.56% in P1 and P2 single culture on the 10 d and 1 d respectively,and 18.83% in P1 and P2 mixed culture on the 2 d.Under different conditions,the degradation rate of insoluble phosphorus was affected to some extent,and the mixed culture at 40℃reached as high as 33.82% on 6 d.P1 mainly produced ace-tic acid,up to 4 103.65 mg/L,P2 mainly produced acetic acid and succinic acid,mixed culture mainly produced acetic acid,followed by oxalic acid.The two strains had strong growth ability in distillers′ grains,which provided a certain reference for preparation of bioorganic fertilizer with distiller′s grains.

phosphate-soluble bacteriaphosphorus solubilityorganic acidsvinassesoil

李秀彤、张迪、赵福培、江友峰、曾祥、王晓丹、程利芳羽

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贵州大学 酿酒与食品工程学院,贵州 贵阳 550025

贵州省发酵工程与生物制药重点实验室,贵州 贵阳 550025

贵州茅台酒厂(集团)循环经济产业投资开发有限公司,贵州 遵义 564500

贵州省白酒制造业创新中心,贵州 仁怀 564500

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解磷菌 解磷能力 有机酸 酒糟 土壤

校企合作项目贵州省省级科技计划项目贵州省省级科技计划项目贵州省省级科技计划项目贵州省省级科技计划项目

K21-0115-017黔科合成果[2021]一般048黔科合成果[2022]重点002号黔科合支撑[2022]一般215黔科合支撑[2021]一般279

2024

微生物学杂志
中国微生物学会 辽宁省微生物学会 辽宁省微生物科学研究院

微生物学杂志

CSTPCD北大核心
影响因子:0.964
ISSN:1005-7021
年,卷(期):2024.44(3)