首页|活化富集对土壤样品微生物菌群组成的影响

活化富集对土壤样品微生物菌群组成的影响

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地球上的微生物绝大多数仍处于未培养状态.微生物的分离培养有助于新物种资源、新天然产物的发现和应用,也有助于对微生物开展生理生化、代谢潜能、演化和生态功能等方面的基础研究.活化富集对于自然生境中微生物的分离培养至关重要.本研究通过16SrRNA基因V3～V4区扩增子测序和宏基因组测序分析了土壤样品不同培养基(WB、PJ、WB/10和PJ-M)、不同活化时长(1～29 d)和不同活化条件(有氧和厌氧)对微生物活化富集的影响.发现活化培养基、培养条件和活化时间的不同会显著影响活化富集后样品的菌群组成.其中,采用营养成分浓度相对较低的培养基(WB/10)、延长活化时长(活化5～29 d)有利于提高活化富集后样品微生物群落的多样性,以及CPR等微生物暗物质的活化及检出.对后续微生物,特别是微生物暗物质的分离培养具有一定的指导作用.
Effect of Activation and Enrichment on Soil Microbial Community Structure
The vast majority of the Earth's microbes remain uncultured.Isolation and cultivation of microorganism are beneficial to the discovery and application of new species resources and new natural products,as well as the develop-ment of basic researches on the physiology and biochemistry,metabolic potential,evolution and ecological function of microorganism.Activation and enrichment are imperative for the isolation and cultivation of microorganism in natural habitats.In this study,the effects of different media(WB,PJ,WB/10 and PJ-M),different activation durations(1-29 d),and different activation conditions(aerobic and anaerobic)on microbial activation and enrichment of soil sam-ples were analyzed by 16S rRNA gene V3-V4 amplicon sequencing and metagenomic sequencing.It was found that the different activation media,cultivation conditions and activation time significantly affected the composition of microbial population.Most of which the use of medium with relatively low nutrient concentration(WB/10)and extension of ac-tivation time(5-29 days of activation)conducive to improve the diversity of activated microorganism,the activation,and the detection as well as CPR and other of microbial dark matter.It has certain guiding effect on the subsequent isolationand cultivation of microorganism,especially microbial dark matter.

activation and enrichmentactivation duration16S rRNA gene V3-V4 amplicon sequencingmet-agenomic sequencingmicrobial dark matter

姜凯、曹春玲、红雨

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内蒙古师范大学生命科学与技术学院,内蒙古呼和浩特 010022

内蒙古自治区高等学校生物多样性保护与可持续利用重点实验室,内蒙古呼和浩特 010022

内蒙古自治区农牧业技术推广中心,内蒙古呼和浩特 010010

活化富集 活化时长 16S rRNA基因V3-V4区扩增子测序 宏基因组测序 微生物暗物质

内蒙古自然科学基金项目内蒙古自治区科技计划项目内蒙古师范大学基本科研业务费项目内蒙古师范大学基本科研业务费项目

2020BS030062020GG00392022JBQN0912022JBTD010

2024

10.3969/j.issn.1005-7021.2024.04.006

微生物学杂志
中国微生物学会 辽宁省微生物学会 辽宁省微生物科学研究院

微生物学杂志

CSTPCD北大核心
影响因子：0.964
ISSN：1005-7021
年,卷(期)：2024.44(4)