Effects of phosphorus solubilizing biological fertilizer on soil nutrients and bacterial community structure
In order to reveal the effects of phosphorus solubilizing biological fertilizer on soil nutrients and bacterial community structure in facilities,phosphorus solubilizing bacteria P623-9 and decomposed chicken manure were com-bined to prepare the phosphorus solubilizing biological fertilizer,the plant soil was used as the research object in the pot experiment,Using pepper as test material which is widely cultivated at greenhouses in the northern,four experi-mental treatments were used:CK:blank control;Y:Chicken manure(decomposed)3000 kg/hm2;SY:Phosphorus solubilizing biological fertilizer 3000 kg/hm2;S:Phosphorus solubilizing bacteria P623-9 240 kg/hm2.Traditional statistical methods were used for statistical analysis of soil nutrient content,The results showed that the contents of AP,TN,MBC,MBN and MBP were increased by SY treated soil,compared with other treatments,the difference was significantly increased(P＜0.05);Illumina Miseq high-throughput sequencing was used to analyze the community structure,abundance and differences of soil bacteria.The results showed that the Actinobacteria,Acidobacteria,Pro-teobacteria,Firmicutes,Chloroflexi,Gemmatimonadetes,and Bacteroidetes were dominant at phylum level.The rela-tive abundance of Proteobacteria,Firmicutes,and Chloromyces was higher shen treated with SY;the relative abun-dance of Bacillus,Arthrobacter,Sphingomonas,and Streptomyces were higher at genus level.The relative abundance of Arthrobacter was increased to 3.95％abundance compared to CK treatment when treated by SY,Beijerinckiaceae was positively correlated with available phosphorus,total nitrogen,microbial phosphorus and microbial nitrogen,while it was negatively correlation with total phosphorus;Nocardioidaceae was significantly positive correlation with microbial nitrogen,however,it was weakly correlation with total nitrogen and available phosphorus;Microscillaceae was posi-tively correlated with total nitrogen,microbial carbon,microbial nitrogen and microbial phosphorus.Draw a conclu-sion,the application of biological phosphorus fertilizer can significantly increased the soil nutrient content,increase the abundance and diversity of beneficial flora.This study revealed the effects of phosphorus solubilizing biological fer-tilizer on soil bacterial community structure,diversity and nutrients,it provided a theoretical basis for market applica-tion and popularization.
万方数据
维普
