首页|次黄嘌呤核苷酸脱氢酶及其促进酿酒酵母核酸的合成

次黄嘌呤核苷酸脱氢酶及其促进酿酒酵母核酸的合成

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核糖核酸(Ribonucleic acid,RNA)的降解产物及其衍生物具有多种生物学功能,属于药食同源,近年来已在新型食品增味剂、保健品、抗病毒制剂及农用增产剂等方面广泛使用,具有较高的市场价值,目前主要通过发酵法培养微生物获得,然后被酶促转化成多种降解物及衍生物,以满足市场需求.酿酒酵母(Saccharo-myces cerevisiae)是公认的食品安全性微生物,且核酸含量较高,是理想的RNA生产微生物.微生物系统工程团队(本团队)在前期的研究中发现,强化次黄嘌呤核苷酸脱氢酶(Inosine monophosphate dehydrogenase,IMP-DH)有利于提高酿酒酵母核糖体RNA(Ribosome RNA,rRNA)的合成.而从医药角度,IMPDH-直被作为研发抗癌、抗病毒和抗寄生虫药物的靶点.本文综述了 IMPDH的研究现状,重点介绍了酿酒酵母IMPDH的生物学功能、结构、同工酶及表达调控机制,并结合IMPDH与rRNA合成的关联,展望了其在提高酿酒酵母核酸产生中的作用,以期为突破核酸产业的技术瓶颈提供理论依据.
Hypoxanthine Nucleotide Dehydrogenase and Its Enhancement of Nucleic Acid Synthesis in Saccharomyces cerevisiae
Ribonucleic acid(RNA)degradation products and their derivatives have many biological functions and be-long to medicinal and food origin.In recent years,they have been widely used in new food flavor enhancers,health products,antiviral preparations,and agricultural yield enhancers etc,and widely used in many aspects,and have fairy high market values.At present,they are mainly obtained by microorganisms through fermentation,then enzymat-ically converted into a variety of degradation products and derivatives to meet market demand.Saccharomyces cerevisi-ae,a generally regarded as safe(GRAS)microorganism with a high nucleic acid content,is an ideal microorganism RNA producer.In the previous study,it was found that enhanced the expression of hypoxanthine nucleotide dehydro-genase(HNDH)induced to the synthesis of ribosome RNA(rRNA)in S.cerevisiae.From a pharmaceutical perspec-tive,HNDH has been used to study as a target for the development of anticancer,antiviral,and antiparasitic drugs.This paper reviews the current research status of HNDH,focusing on the biological function,structure,isozyme,and expression regulation mechanism of HNDH.Combined HNDH association with rRNA synthesis,its role in enhancing nucleic acid production in S.cerevisiae prospects.So as to provide a theoretical basis for breaking the technical bottle-neck of the nucleic acid industry.

hypoxanthine nucleotide dehydrogenase(HNDH)guanosine triphosphateexpression regulationnucle-ic acidsribosomeSaccharomyces cerevisiae

王赟、夏天晴、徐丽丽、李在禄、鲍晓明

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齐鲁工业大学(山东省科学院)生物基材料与绿造纸国家重点实验室生物工程学院,山东济南 250353

次黄嘌呤核苷酸脱氢酶 鸟嘌呤核苷三磷酸 表达调控 核酸 核糖体 酿酒酵母

国家自然科学基金项目山东省自然科学基金项目山东省重点研发计划齐鲁工业大学(山东省科学院)科教产重大创新专项

31801515ZR2019PC0102017CXGC11052022JBZ01-06

2024

10.3969/j.issn.1005-7021.2024.04.011

微生物学杂志
中国微生物学会 辽宁省微生物学会 辽宁省微生物科学研究院

微生物学杂志

CSTPCD北大核心
影响因子：0.964
ISSN：1005-7021
年,卷(期)：2024.44(4)