The CRISPR-Cas system has revolutionized genetic modification in both prokaryotic and eukaryotic micro-organisms,boosting the capabilities of industrial cell factories.Notable strains like Escherichia coli,Bacillus subtilis and Corynebacterium glutamicum in prokaryotes,and Saccharomyces cerevisiae,Pichia pastoris and Yarrowia lipolytica in eukaryotes,have undergone metabolic network reconstruction and precise gene editing,enhancing production effi-ciency.Advanced tools like CRISPR-Cas9,CRISPR-Cas12a and base editors have streamlined metabolic pathway op-timization.High-throughput editing technologies such as CHASE,CREATE and iCREATE in conjunction with CRISPR-Cas,allow for simultaneous multi-gene editing,speeding up the development of complex phenotypes and met-abolic network analysis.This summary highlights the CRISPR-Cas system's role in microbial modification and its po-tential in shaping the future of industrial microbial cell factory construction.
维普
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