Optimization of Polyphenols Extraction Techonology from Red Volva of Dictyophora rubrovalvata by Response Surface Methodology and Its Antioxidant Activity
Optimization of Polyphenols Extraction Techonology from Red Volva of Dictyophora rubrovalvata by Response Surface Methodology and Its Antioxidant Activity
In order to evaluate the polyphenolic compounds and achieve the resource utilization of red volva of Dictyo-phora rubrovalvata,a kind of edible mushroom grows in summer bamboo groves.First,a single factor test was carried out on four extraction conditions ( ethanol volume fraction,ultrasonic time,extraction temperature and material liquid ratio),then response surface method was used to optimize the extraction conditions.Meanwhile,the antioxidant activ-ity of volva polyphenols were analyzed.The result showed that to obtain polyphenol extraction using optimal technology conditions from volva of D.rubrovalvata were:Ethanol volume fraction 50％,ultrasonic time 16 minutes,extraction temperature 54 ℃ and material-liquid ratio at 1︰15 g/mL.At these optimized extraction conditions,the actual ex-traction amount of polyphenols could reach 9.15 mg/g.The volva polyphenols exhibited preferably antioxidant activi-ty.When the mass concentration of volva polyphenols was 0.80 mg/mL,the total antioxidant capacity reached to 0.643 Trolox μmol/mL.The clearance rates of diphenyl picryl hydrazinyl ( DPPH) radicals,hydroxyl free radicals and superoxide anion free radicals were 71.45％,79.02％ and 60.38％ respectively.To conclude:Volva of D.rubrovalvata is riched in polyphenolic compounds and have good antioxidant activity.The optimization of polyphenols extraction technology of volva from D.rubrovalvata could provide reference for resource utilization.
关键词
红托竹荪(Dictyophora/rubrovalvata)/菌托/多酚/提取工艺/抗氧化活性
Key words
D. rubrovalvata/volva/polyphenols/extraction technology/antioxidant activity
维普
万方数据