Isolation and Identification of Culturable Midgut Bacteria of Culicoides arakawae in Jiangcheng County in Yunnan Province
Culicoides arakawae is a common blood-sucking insect in chicken farms which mainly spreads a variety of poultry diseases,such as leucocytozoonosis and avian pox.To understand the culturable midgut bacteria species and community composition of culturable midgut bacteria of C.arakawae,to provide foundation for early stage work re-search on the influence of midgut bacteria of C.arakawae on disease transmission.C.arakawae collected in Jiangcheng County were used as material to isolate and identify the culturable midgut bacteria.Culicoides were collect-ed using light traps,and non-blood-fed female specimens of C.arakawae were selected.The midgut was dissected and ground,and the culturable bacteria were isolated from the midgut,and identified and analyzed by 16S rDNA se-quencing.The results showed that a total of 8419 culturable midgut bacteria were obtained from the midgut of 4 C.arakawae,and 38 strains were purified,belonging to 10 species of 6 genera,5 families,and 2 phyla.The domi-nant bacteria were the gram-positive bacterium,accounting for 57.89％ of the total isolated strains,and the dominant strains were Staphylococcus,accounting for 55.26％ of the total isolated strains.Culturable midgut bacteria of C.ara-kawae in Jiangcheng county belong to Proteobacteria and Firmicutes,with Staphylococcus as the main species.
万方数据
维普
