Characteristics of Fungal Community and Analysis on Their Responses to Environmental Factors in Sediments of Longjiang River
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维普
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鉴于龙江宜州段曾受重金属镉污染,为探索污染流域龙江宜州段上下游的河流沉积物中环境因子对真菌群落多样性的影响,采用高通量测序方法对龙江宜州区段的五个样区沉积物样本的真菌群落组成和多样性进行比较分析,并结合相关性分析方法研究真菌群落与理化性质及重金属参数的关系.经测序,质控获得7027个分类操作单元(OTUs),隶属17门、52纲、125目、295科、622属.各样区占比较大的是未分类真菌门类(unclassified k Fungi),范围在43.6％～56.8％,占比最大在河流中段.优势菌门主要有罗兹菌门(Rozello-mycota)、子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、壶菌门(Chytridiomycota).相关性分析表明,担子菌门、被孢霉门(Mortierellomycota)、毛霉门(Mucoromycota)等受重金属铜(Cu)极显著正向调控(P<0.01),且担子菌门与速效磷(AP)及重金属镉(Cd)呈极显著负相关关系(P<0.01).另外AP对芽枝霉门(Blastocladio-mycota)、蛙粪菌门(Basidiobolomycota)呈极显著正相关,且对沉积物真菌多样性结构显著正相关影响,而真菌群落丰富度受铜(Cu)和锌(Zn)显著相关性影响.通过研究流域比较分析发现,河流中段沉积物真菌群落多样性及物种组成与上下游存在差异,AP和重金属Cu、Zn、Cd含量是龙江沉积物真菌群落结构变化的主要生态因子,本研究为龙江流域生态评估和后期耐受重金属真菌开发提供参考.
Considering once cadmium contaminated in the Yizhou section of the Longjiang River,the effects was ex-plored the characteristics of fungal community structure and its correlation with environmental factors in sediments in the upper and lower reaches of Yizhou section of Longjiang River.The diversity and composition of fungal community in the surface sediments of 5 sampling points were analyzed by diversity comparison using high-throughput sequencing,and the relationship between fungal community and parameters of physicochemical property and heavy metals was stud-ied by reciprocity analysis.After sequencing a total of 7027 OTUs,belonging to 17 phyla,52 classes,125 orders,295 families and 622 genera were obtained.The proportion of unclassified fungi ranged from 43.6％ to 56.8％ in the sampling points,with the largest proportion in the midsection of the Longjiang River.On the phylum level,the domi-nant fungi in the sediments mainly include Rozellomycota,Ascomycota,Basidiomycota,and Chytridiomycota.The person correlation analysis found that Basidiomycota,Mortierellomycota and Mucoromycota were positively regulated by Cu (P<0.01),and Basidiomycota negatively correlated with Olsen phosphorus (Olsen-P) and Cd (P<0.01).In addition,Olsen-P was positively regulated the Blastocladiomycota and Basidiobolomycota,which also was positively correlated with the diversity of fungal community in the sediments,the fungal richness was significantly correlated with heavy metals,such as Cu and Zn.Compared with the samples collected in the sediments of Longjaing River,it was found that the diversity and species composition of fungal community in the sediments of the middle reaches of the river were different from those in the upper and lower reaches,the content of Olsen-P and heavy metals Cu,Zn,Cd were the main ecological factors for the variation of fungal community structure,which would provide a scientific basis for ecological assessment and development of heavy metal tolerant fungi in the Longjiang River.
维普
万方数据
