Compensatory changes of cerebral venous blood flow in the very early stage of cerebral venous and sinus thrombosis
Objective:To investigate the compensatory status and characteristics of venous blood flow in the very early stage of cerebral venous and sinus thrombosis(CVST).Methods:Twelve SD rats were randomly divided as control group and embolization group.In the embolization group,an SD rat superior sagittal sinus(SSS)embolization model was constructed using the thread embolization method.While observing the anatomical characteristics and course of cerebral surface veins,laser speckle flow imaging system and small animal MRI technology were combined to observe the superficial veins themselves and the collateral circulation between deep veins in the early stage of SSS embolization.At 24 hours,venous blood was drawn from two groups of SD rats,and the levels of claudin-5 and ZO-1 in the serum were detected by ELISA method.At the same time,the rats were euthanized and brain tissues were taken.The infarct volume of the two groups of SD rats was measured using 2,3,5-triphenyltetrazolium chloride(TTC)staining method,and the ultrastructure of tight junction protein in endothelial cells was observed using electron transmission microscopy.Results:In the case of SSS acute embolism,extensive collateral circulation between the superficial veins themselves and the deep veins was rapidly formed on the surface of the brain in the embolization group of SD rats,and the serum claudin-5 concentration in the embolization group was higher than that in the control group.Although partial opening of tight junction proteins in endothelial cells was observed under electron transmission microscopy,no obvious venous cerebral infarction was found in TTC staining.Conclusion:After acute embolization of SSS,cerebral venous collateral circulation can provide sufficient compensation to control venous ischemic brain injury within a certain limit,which gives a reasonable explanation for the hidden pathogenesis and complex clinical manifestations of CVST.
embolism of superior sagittal sinuscerebral venous blood flowcollateral circulationvenous cerebral infarctionrats
万方数据
维普
