硫酸胆固醇对桥本氏甲状腺炎小鼠模型的治疗效果及相关机制研究
Therapeuti ceffect and potential mechanism of cholesterol sulfate on a mouse model of Hashimoto's thyroiditis
何林烨 1邱焕迪 2何巍 3胡以国 1李志辉4
作者信息
- 1. 四川大学华西医院甲状腺外科,四川成都 610041;四川大学生物治疗国家重点实验室,四川成都 610041
- 2. 四川大学华西医院分子医学研究中心,四川成都 610041
- 3. 四川大学生物治疗国家重点实验室,四川成都 610041
- 4. 四川大学华西医院甲状腺外科,四川成都 610041
- 折叠
摘要
目的 观察硫酸胆固醇(CS)对桥本氏甲状腺炎小鼠的治疗效果.方法 选取NOD.H-2h4雌性小鼠饮0.05％碘化钠水持续不同时间,并通过持续2周腹腔注射CS对诱导小鼠进行治疗.HE染色观察淋巴细胞浸润情况并进行甲状腺炎症程度评分;测定血清抗甲状腺球蛋白抗体(TgAb)、甲状腺过氧化物酶抗体(TPOAb)以及甲状腺功能水平;免疫荧光染色流式细胞仪分析B淋巴细胞、调节性T细胞(Treg)、Th17、Th1、Th2细胞比例的变化.结果 HE染色结果显示,在8周组和16周组中,经过CS的腹腔注射后,小鼠甲状腺组织炎性评分显著下降(P＜0.05).而在64周组中,治疗组与诱导组之间无显著差别(P=0.31).血清学分析显示,经过CS干预后,高碘溶液诱导8周和16周的小鼠体内TgAb、TPOAb水平均显著降低(P＜0.05),而小鼠的甲状腺功能(TSH、T4水平)仅在16周组出现显著变化.流式细胞术分析显示,在8周组中,经过CS干预后,小鼠体内B淋巴细胞以及Th1、Th2、Th17和Treg细胞比例均出现显著变化(P＜0.05).结论 CS对于桥本氏甲状腺炎早中期的疾病进展具有明显的治疗及缓解效果.
Abstract
Objective To investigate the therapeutic effect of cholesterol sulfate(CS)on the Hashimoto's thyroiditis mouse model.Methods Female NOD.H-2h4 mice were fed with 0.05％NaI in different periods and treated with CS by intraperitoneal injection for two consecutive weeks.HE staining was used to visualize and score the degree of lymphocyte infiltration in the thyroid;serum levels of thyroglobulin antibody(TgAb),thyroid peroxidase antibody(TPOAb),thyroxine(T4),and thyroid stimulating hormone(TSH)were detected by ELISA.The proportions of B cells and Treg,Th17,Th1,and Th2 cells were analyzed by immunofluorescence staining flow cytometry.Results HE staining showed that the inflammatory score of thyroid tissue in mice after intraperitoneal injection of CS in the 8-week group and the 16-week group decreased significantly(P＜0.05).In the 64-week group,there was no significant difference between the treatment group and the induction group(P=0.31).Serological analysis showed that after CS intervention,the levels of TgAb and TPOAb in mice induced by 0.05％NaI significantly lowered(P＜0.05)in the 8-week group and the 16-week group,while thyroid function(TSH and T4 levels)of the mice changed significantly only in the 16-week group.Flow cytometry analysis showed that in the 8-week group,after CS intervention the proportions of B lymphocytes and Th1,Th2,Th17 and Treg cells in mice were significantly changed(P＜0.05).Conclusion CS has significant therapeutic and remission effects on the early and middle stages of Hashimoto's thyroiditis.
关键词
NOD.H-2h4小鼠/桥本氏甲状腺炎/硫酸胆固醇/调节性T细胞/Th17细胞Key words
NOD.H-2h4 mouse/Hashimoto's thyroiditis/cholesterol sulfate/regulatory T cell/T helper 17 cell引用本文复制引用
基金项目
四川省自然科学基金青年科学基金(2023NSFSC1880)
四川省科技厅重点研发项目(2023NSFSC1880)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据