弹性蛋白酶诱导的小鼠腹主动脉瘤消退期组织学特征观察
Histological characteristics of elastase-induced mouse abdominal aortic aneurysm in regression stage
李濛 1刘浩乐 2魏盼盼 2李可心 2吴海滨 2侯海雯 2刘恩岐 2李艳奎 1赵四海2
作者信息
- 1. 天津医科大学第二医院血管外科,天津 300211
- 2. 西安交通大学医学部实验动物中心,陕西西安 710061
- 折叠
摘要
目的 确定猪胰弹性蛋白酶(PPE)诱导的腹主动脉瘤(AAA)到达消退期的时间点,观察AAA消退期的组织学特征.方法 选用C57BL/6J小鼠,手术游离腹主动脉,进行血管腔内输注PPE诱导AAA.测量小鼠术前、术后14 d(AAA到达平台期的时间点)和术后56 d(AAA消退期时间点)腹主动脉直径,比较分析术后14 d和术后56 d动脉瘤病变部位组织学特征.结果 与正常腹主动脉相比,PPE输注14 d组和56 d组,腹主动脉直径均显著增加(直径增长率分别为147％和155％).PPE输注后14 d,动脉瘤病变部位显微镜镜下可见弹性纤维断裂、平滑肌耗竭和炎症细胞弥散浸润等典型的AAA特征.在PPE输注后56 d组,虽然动脉直径没有明显变化,但组织学可见弹力纤维部分修复,新生平滑肌细胞补充至损伤的中膜,浸润的炎症细胞明显消退,外膜新生血管减少,呈现出疾病消退期的显著特征.结论 在PPE诱导的小鼠AAA模型中,术后56 d是观察动脉瘤消退的合适时间点,动脉瘤病变消退的组织学特征明显.
Abstract
Objective To determine the time point when porcine pancreatic elastase(PPE)induced abdominal aortic aneurysm(AAA)reaches the regression phase in mice and observe the histological characteristics of AAA in regression phase.Methods AAAs were induced by transient intraluminal infusion of PPE in C57BL/6J mice.The diameters of the mouse abdominal aortas were measured before PPE infusion and sacrifice time,day 14 for AAA progression phase or day 56 for regression phase after PPE infusion,respectively.The histological characteristics of the aneurysm lesion site on day 14 and day 56 after surgery were compared and analyzed.Results The diameters of the abdominal aortas were significantly increased in both day 14 and day 56 after PPE infusion groups(diameter growth rate 147％and 155％,respectively)as compared to the baseline diameters.In the day 14 group,the infused aortas showed typical AAA characteristics,such as elastin break/degradation,medial smooth muscle cells depletion,and inflammatory cell diffused infiltration.In the day 56 group after PPE infusion,although the artery diameter did not change significantly as compared to the day 14 group,histology showed that elastin was partially repaired,new smooth muscle cells were added to the damaged aorta media,the infiltrated inflammatory cells were significantly subsided,and the adventitia neovascularization was reduced,showing a significant feature of the disease regression phase.Conclusion In the PPE-induced mouse AAA model,day 56 after surgery is an appropriate time point for observing aneurysm regression,and the histological characteristics of the regression are obvious.
关键词
腹主动脉瘤/消退/弹性蛋白酶/小鼠/组织学Key words
abdominal aortic aneurysm/regression/elastase/mouse/histology引用本文复制引用
基金项目
陕西省自然科学基金(2023-CX-PT-17)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据