The killing effects of gentamicin on uropathogenic Escherichia coli and its cytotoxicities in in vitro experiments of urinary tract infection
Objective To compare the killing effects of different concentrations of gentamicin(0,10,20,50,100,and 200 μg/mL)on uropathogenic Escherichia coli(UPEC)and its cytotoxicities on urinary urothelial cells and inflammatory cells such as macrophages in vitro.Methods The killing effects of different concentrations of gentamicin on different amounts(108,107,and 106)of UPEC strain J96 were compared.The cytotoxicities of different concentrations of gentamicin on primary cultured male C57BL/6 mouse renal tubular epithelial cells,mouse macrophages and human bladder epithelial cell line J82 at different time points(2 h and 24 h)were detected by CCK-8 assay.According to the experiments above,we chose appropriate gentamicin concentrations and incubation time in in vitro cell culture experiments to verify J96 adhesion and invasion to mouse renal tubular epithelial cells or phagocytosis and clearance of J96 by mouse macrophages.Results The killing effect of gentamicin(≥10μg/mL)on J96 was stronger than that of 1％P/S(P＜0.000 1).High concentrations of gentamicin(≥100 μg/mL)could kill up to 108 J96 within 30 min.50 μg/mL gentamincin treatment for 2 h was cytotoxic for human bladder epithelial cell line J82(P＜0.05).Conclusion The appropriate concentration and duration of gentamicin treatment for different cells in vitro were determined.Urothelial cells,especially human bladder epithelial cell line J82,were more sensitive to gentamicin.
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维普
