Investigation of adsorption of human serum albumin on gold surface by zero current potentiometry
Aim To investigate the adsorption behavior of human serum albumin (HSA) on the gold surface in pH 7.0 phosphate buffer solution. Methods A novel electrochemically approach, zero current potentiometry, was used. Results The adsorption of HSA on the gold surface resulted in the gradual shifts of the recorded 1-E curve and zero current potential Elcp along potential axis in negative direction. The plot of zero current potential Etxp a-gainst adsorption time t showed that the adsorption of HSA on the gold surface involved two steps, adsorption and conformation change of HSA. And the apparent rate constant kobs the adsorption obtained was (4. 0 ± 1. 8) × 10-3 s-1 Conclusion Using zero current potentiometry, the adsorption of HSA on the gold surface can be probed in situ and continuously, without the destruction of the formed adsorption layer.
Human serum albuminGoldAdsorptionZero current potentiometry
NETL
NSTL
万方数据
维普
