Relationship Between Tissue Browning and the Contents of Phenolics and Related Enzymes Activity in Peaonia suffruticosa
The dynamic changes of the contents of phenolic and the activities of phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD) were assessed during the differentiation process of three tree peony (Peaonia suffruticosa) cultivars Cwu Long Peng Sheng', 'Feng Dan Bai' and 'Tai Ping Hong') grown in vitro. A close relationship were observed between the tissue browning and the activities of phenylalanine ammonia lyase (PAL), polyphenoloxidase (PPO), and peroxidase (POD) activity, as well as phenolic contents during the differentiation process in tree peony in vitro, the higher browning degree of the cultivars, the lower phenolic contents and POD activity, and the higher PPO activity. The similar pattern was found between PAL activity and tree peony browning.