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牡丹组培褐变与总酚含量及相关酶活性的关系

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以3个褐变程度不同的牡丹品种(‘乌龙捧盛’、‘太平红’、‘凤丹白’)为材料,分析了试管苗在增殖过程中总酚含量、苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)和过氧化物酶(POD)活性的动态变化.结果表明:总酚含量及PPO、POD、PAL活性与牡丹试管苗增殖过程中的褐变密切相关.褐变越严重的品种,总酚含量和POD活性越低,PPO活性越高,PAL活性与牡丹的褐变程度未能表现出类似的规律.
Relationship Between Tissue Browning and the Contents of Phenolics and Related Enzymes Activity in Peaonia suffruticosa
The dynamic changes of the contents of phenolic and the activities of phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD) were assessed during the differentiation process of three tree peony (Peaonia suffruticosa) cultivars Cwu Long Peng Sheng', 'Feng Dan Bai' and 'Tai Ping Hong') grown in vitro. A close relationship were observed between the tissue browning and the activities of phenylalanine ammonia lyase (PAL), polyphenoloxidase (PPO), and peroxidase (POD) activity, as well as phenolic contents during the differentiation process in tree peony in vitro, the higher browning degree of the cultivars, the lower phenolic contents and POD activity, and the higher PPO activity. The similar pattern was found between PAL activity and tree peony browning.

Paeonia suffruticosabrowningphenol contentenzyme activity

符真珠、陈静、徐盼盼、何松林、王政

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河南农业大学林学院,河南郑州450002

牡丹 褐变 总酚 酶活性

国家自然科学基金郑州市科技创新团队项目农业部“948”项目

3107181610CXTD1472009-Z28

2011

西北林学院学报
西北农林科技大学

西北林学院学报

CSTPCDCSCD北大核心
影响因子:1.078
ISSN:1001-7461
年,卷(期):2011.26(6)
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