Studied on Changes Hormone in the Periods of Making Roots in the Softwood of Robinia pseudoacacia
In order to understand the causes of the difficulty in rooting,current year soft wood cuttings of tetraploid locust were treated by an exogenous hormone,IBA with different concentrations and time of treatment to examine their effects on rooting and the levels of endogenous hormones,such as IAA,adenine,ABA,GA,and IBA.The results showed that:1) During the rooting process,contents of IAA,adenine and GA in the cuttings increased in the first 0-15 days,but decreased during 15-30 days.Adventitious roots formation required high IAA level,and then IAA synthesis weakened in order to maintain the normal growth.2) No significant correlation was observed between adenine content and callus induction rate,certain amount of adenine was needed to promote callus induction and differentiation before the formation of adventitious root (0-15 d).3) Significantly negative correlation was found between GA content and callus induction rate,and the increase of the levels of GA before adventitious root formation was likely to be the main reason for inhibition of adventitious root formation.4) ABA and IBA contents in softwood cuttings had no significant correlation with callus induction rate.