Establishment of Plantlet Regeneration System of Idesia polycarpa in vitro
Seeds and tender leaves of Idesia polycarpa were used as a source of initial explants.The effects of explants and plant growth regulators on organogenesis and plantlet regeneratio in vitro were studied.The results showed that seeds were more suitable for initial explants in inducing calli.High induction rate of 96.8％ was achieved in MS medium supplemented with 1.0 mg· L-1 NAA,1.0 mg· L-1 6-BA,3％ sucrose and 0.6 ％agar.MS medium supplemented with 0.08 mg · L-1 TDZ,1.5 mg · L-1 6-BA and 0.05 mg · L-1 NAA in subculture was in favor of the shoot differentiation and the rate was over 48.3％.The shoot proliferation coefficient reached 4.83 in 1/2MS medium with 0.03 mg · L-1TDZ,2.0 mg · L-1 6-BA,0.1 mg · L-1 NAA,3％ sucrose and 6％ agar.High rooting percentages and excellent development root systems were obtained on 1/2MS medium with 0.5 mg · L-1 IBA,2％ sucrose and 0.6％ agar.
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