首页|Folin-Ciocalteu法测定大熊猫主食竹中多酚含量

Folin-Ciocalteu法测定大熊猫主食竹中多酚含量

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
为建立Folin-Ciocalteu法测定大熊猫主食竹缺苞箭竹(Fargesia denudata)总酚酸含量的方法.以没食子酸为对照品,研究了Folin-Ciocalteu法测定缺苞箭竹中酚酸类物质含量的适宜条件.结果表明,适宜的比色条件为Folin-Ciocalteu试剂与10％ Na2CO3体积比为1∶6,Folin-Ciocalteu试剂3 mL,显色温度25℃,反应时间2h,检测波长780 nm.浓度为20～100 μg·mL-1时与其吸光值呈良好的线性关系,测定方法的平均回收率为99.4％.可见该法有较好的准确度和重现性.
Determination of the Contents of Polyphenols in Fargesia denudata, the Main Food of Giant Penda by Folin-Ciocalteu Colorimetric Method
Aims:to establish a quantitative method of measuring total phenols in Fargesia denudata,the main food of giant panda.Methods:with gallic acid as reference substance to study the suitable conditions of the measurement by Folin-Ciocalteu method.Results:the optimum conditions for determining total polyphenols were as follows:the volume ratio of Folin-Ciocalteu reagent to 10％ Na2CO3:1 ∶ 6,3 mL of Folin-Ciocalteu reagent incubated at 25℃for 2 h,followed by detection at 780 nm.Good linear relationship was found in the concentration of 20-100μg · mL-1,the average recovery was 99.4％.

Fargesia denudataFolin-Ciocalteuployphenol

赵莘、段丽娟、高健、郑丽丽、李俊清

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北京林业大学省部共建森林培育与保护教育部重点实验室,北京100083

缺苞箭竹 Folin-Ciocalteu法 总酚

林业公益性行业科研专项

201404422

2014

10.3969/j.issn.1001-7461.2014.06.40

西北林学院学报
西北农林科技大学

西北林学院学报

CSTPCDCSCD北大核心
影响因子：1.078
ISSN：1001-7461
年,卷(期)：2014.29(6)
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