Biochemical Characteristic Variations of Embryogenic and Non-embryogenic Callus of Quercus variabilis
Somatic embryogenesis is a complex process.In order to reveal the regeneration potential of embryogenic callus,this paper investigated the difference of some biochemical characteristics,including soluble sugars,soluble proteins,starch,free proline,phenolics and proteolytic activity in embryogenic and non-embryogenic callus induced by immature zygotic embryos of Quercus variabilis.The contents of soluble sugars,soluble proteins,starch,free proline in embryogenic callus were 97.6,25.3,28.3,and 202.8mg · g-1,respectively,more than those in non-embryogenic callus;while proteolytic activity in two calli had no significant difference.However,non-embryogenic callus had a higher phenolics level compared with embryogenic callus.