Aging and Rejuvenation Pattern of Reproductive Material of Populus tomentosa Sprouts
The regeneration of Populus tomentosa mainly relys on asexual reproduction,but the material of asexual propagation often has aging phenomenon.It is important to understand the aging pattern of reproductive material and the rejuvenation techniques.In this study,the stump sprouts were collected from 200-(mature) and 30-year-old (younger) trees of male P.tomentosa root to study the aging pattern and rejuvenation technique.Relative parameters were measured by using the established aseptic regeneration systems,such as subculture cycle,bud proliferation during subculture,and rooting rate.Compared with the stump sprouts collected from younger tree,the survival rate of the sprouts of mature tree was 29％ lower,the growth cycle was 40 days longer,and the effective proliferation decreased by 13％.After successive transfer culture 3 generations,the proliferation coefficient of the sprout of mature tree increased from 0.93 to 3.77,the percentage of plant number(stem increment≥3 cm)increased from 2.23％ to 38.3％.For the younger tree,after successive transfer culture 6 generations,the proliferation coefficient increased from 3.11 to 6.81,the percentage of plant number(stem increment≥3 cm)increased from 26.7％ to 51.3％.Trees of different ages had rejuvenation effect after generations of breeding,but the effect among different generations of 200-year-old tree were significantly different (P＜0.05).It was true that there was an aging phenomenon in the propagation material of P.tomentosa,even though the youngest part of the individual development of the tree was selected,the maternal plant age might decide the aging degree of root sprout.The rejuvenation effect of mature trees was much obvious than young trees.
国家科技期刊平台
NSTL
万方数据
维普
