Effects of Exogenous Calcium on Seedling Physiological Characteristics and Endogenous Hormone of Eucalyptus urophylla × E.grandis under Low Temperature Stress
Exogenous Ca2-with different concentrations were sprayed on the 1-year-old seedlings of Eucalyptus urophylla × E.grandis,and the effects of exogenous Ca2-on MDA,osmotic adjustment substances (proline,soluble sugar),activities of antioxidant enzymes (CAT,SOD,POD) and endogenous hormones (CTK,GA,and ABA) were researched under the stress of 4℃.The biomass and CTK,ABA and GA contents of the seedlings decreased significantly and the contents of MDA,proline and soluble sugar increased dramatically under cold stress compared to the treatment of normal temperature.Compared with the absence of calcium,the calcium significantly reduced the contents of MDA,ABA levels and dramatically increased the contents of proline,soluble sugar,CTK,and GA and the activities of CAT,POD,SOD under low temperature stress.The addition of 10 mmol · L-1 CaCl2 showed a better effect than other concentrations of calcium.The seedlings growth under low temperature stress was restrained.The appropriate concentration (10-20 mmol · L-1) of calcium reduced the harm of low temperature on seedlings,meanwhile exogenous calcium enhanced the cold tolerance of the seedlings.
Eucalyptus grandiscalciumlow temperature stressantioxidant enzymeendogenous hormone