Screening and Optimization of Culture Medium and Conditions of Lysurus mokusin
Lysurus mokusin extract has insecticidal and antifungal activities,and it is necessary to cultivate a large number of mycelium because its mycelia grow slowly in the laboratory environment.In order to improve the growth rate of Lysurus mokusin mycelia,the culture medium and conditions were screened and optimized in this study.The culture medium was screened with the growth rate of mycelium as the evaluation index,and the suitable medium for the growth of L.mokusin mycelia was selected out.And on this basis,the carbon source,nitrogen source,inorganic salt addition,pH and culture temperature were further optimized.The best culture medium was 200 g of potato,20 g of glucose,3 g of peptone,10％ inorganic salt solution (KH2PO4 1 g,K2HPO4 1 g,MgSO4 0.5 g,CaCl2 0.05 g,KCl 0.5 g,FeSO4 0.01 g and NaNOa 0.5 g dissolved in 100 mL distilled water) and 15 g of agar.The optimum culture conditions were pH=5 and temperature of 20℃.Under the above culture conditions,the net growth rate of L.mokusin mycelia was 0.59 cm/d,which was 23.69％ higher than that of medium A.
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