SSR-Based Hybrid Identification and Genetic Relationship Analysis of F1 Hybrid Progenies of Lycium ruthenicum
The objective of this study was to identify and analyze the genetic relationship of F1 hybrid based on SSR markers to provide theoretical support for the construction of genetic map,QTL mapping,and the breeding of new cultivars with high anthocyanin content.Taking F1 population'Lr-GA'and'Lr-GB'derived from Lycium ruthenicum and L.ruthenicum'Baiguo'as materials,11 highly polymorphic SSR primer pairs were used to identify the authenticity of F1.Genetic diversity and similarity coefficient were then analyzed.A total of 10 abnormal genotype plants were amplified in 2 F1 progenies using 11 pairs of SSR primers,and identified as false hybrids.The remaining 397 progenies were proved to be real hybrids,the real hybrid rate was 96.81%.Chi-square test revealed only one deviated locus(ML0116,P<0.050),the remaining 10 loci were in accordance with Mendel's law of segregation.In the two hybrids of reciprocal crosses,64 and 48 alleles were detected by 11 pairs of SSR markers,respectively.The number of alleles per marker(Na)ranged from 2 to 9,the effective number of alleles(Ne)ranged from 1.55 to 4.35,the range of Shannon's information index(I)was 0.58 to 1.53,the observed heterozygosity(Ho)ranged from 0.45 to 1.00,the expected heterozygosity(He)ranged from 0.35 to 0.77,and the range of F-test values was-0.58 to 0.22.The range of gene polymorphism information content(PIC)was 0.30 to 0.73,with an average value of 0.59.LM0069 and LM0101 has moderate polymorphisms(0.25<PIC<0.5),while other markers were high and with strong detection ability(PIC>0.5).According to the result of UPGMA clustering,the'Lr-GA'progenies was divided into two groups at the Nei's genetic distance of 0.214,'L.ruthenicum'was Group I and'L.ruthenicum'Baiguo'and 293 F1 individuals were clustered in GroupⅡ.'Lr-GB'population was divided into two groups at Nei's genetic distance of 0.29,'L.ruthenicum'was Group I and'L.ruthenicum'Baiguo'and 101 F1 individuals clustered in Group Ⅱ.Overall,most SSR loci of progenies were variant after hybridization,and the more genetic information derived from the maternal plants than paternal plants.The SSR molecular marker method can identify the F1 hybrids of'L.rutheni-cum'effectively.The genetic separation ratio of F1 hybrids complies with Mendel's law of segregation,and they have close genetic relationship with maternal parent.
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