'Junzao'jujube(Ziziphus jujuba'Junzao')is a dominant dry jujube cultivar in China,the effi-cient rapid propagation technology has not yet been established well.In this paper,focused on the related issues of axillary bud proliferation,subculture,selection of rooting medium,and mycorrhization of rooted seedlings from tissue culture,we tried to establish an efficient and stable propagation technique.The results showed that adventitious buds could be propagated effectively on a modified MS supplemented with 1.2 mg/L 6-BA+0.2 mg/L IBA+0.012 mg/L TDZ.The shoot could be sub-cultured on modified MS supplemented with 1.2-1.5 mg/L 6-BA+0.2 mg/L IBA.Root could be induced efficiently on the optimal rooting medium(1/2MS+IBA 0.6 mg/L+sucrose15 g/L),the root induction rate of shoots was up to 86.89%.Transplanting rooting seedlings into pots containing arbuscular mycorrhizal agents promoted seedling growth significantly compared with non-inoculated seedlings,the survival rate reached 96.30%,18.52%higher than that of the control.The average height of inoculated seedlings was 8.1 cm,1.59 times higher than that of the control.In conclusion,the rapid propagation system in vitro and mycorrhizal seed-ling technology have been established manifested by the features of high proliferation rate,high survival rate after transplantation,and high seedling growth rate,from which a large number of healthy jujube seed-lings can be produced.The results provide technical support for large-scale jujube seedling cultivation.
维普
万方数据