Study on the Content Detection Method of 3 Vanillins and 6 Coumarins in Edible Vegetable Oil
To establish a liquid chromatography-tandem mass spectrometry(LC-MS/MS)method for simultane-ous detection of 3 vanillins and 6 coumarin compounds in edible vegetable oil by optimizing pre-treatment and exploring mass spectrometry conditions,and to determine the feasibility and accuracy of the method.The samples were extracted by acetonitrile ultrasound,purified by HLB column,and re dissolved by nitrogen blowing.Quali-tative analysis was conducted using a multi reaction monitoring positive ion mode,quantitative analysis was per-formed using vanillin internal standard method,and quantitative analysis was performed using coumarin external standard method.The results show that nine target compounds ranging from 20 to 800 μg/L The linearity is good within the range of g/L concentration,and the correlation coefficient(r2)is 0.995 5-0.999 9.The quantitative limit is all 20 μg/L The recovery rates of the 9 compounds obtained by the method are all between 80%and 120%,g/kg.This method has high sensitivity and accuracy,and can be used for the simultaneous detection of 3 vanillins and 6 coumarins in edible vegetable oils.
vanillin compoundscoumarin compoundsliquid chromatography tandem mass spectrometrycon-tent detection
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