粮食加工2024,Vol.49Issue(4) :21-28.DOI:10.20170/j.cnki.lsjg.1007-6395.20240406

响应面优化"漯紫薯4"花色苷提取工艺及其抗氧化活性研究

Optimization of Extraction Process for"Luozishu 4"Anthocyanins by Response Surface Methodology and Their Antioxidant Activity

李轲 芦荣华 张杰华 孟凡奇 仝润喆
粮食加工2024,Vol.49Issue(4) :21-28.DOI:10.20170/j.cnki.lsjg.1007-6395.20240406

响应面优化"漯紫薯4"花色苷提取工艺及其抗氧化活性研究

Optimization of Extraction Process for"Luozishu 4"Anthocyanins by Response Surface Methodology and Their Antioxidant Activity

李轲 1芦荣华 1张杰华 1孟凡奇 2仝润喆3
扫码查看

作者信息

  • 1. 漯河职业技术学院,河南 漯河 462002;河南工业大学漯河工学院,河南 漯河 462002
  • 2. 漯河农业科学院,河南 漯河 462000
  • 3. 河南工业大学漯河工学院,河南 漯河 462002;浙江科技大学,杭州 310023
  • 折叠

摘要

以"漯紫薯 4"为材料,在单因素试验的基础上,采用响应面法对紫薯花色苷的提取工艺进行了优化试验,并对紫薯花色苷的抗氧化活性进行了研究.结果表明,3 个单因素对花色苷得率影响大小的顺序为盐酸体积分数>料液比>提取时间,紫薯花色苷最佳提取工艺参数为盐酸体积分数 0.75%、料液比 1∶20(g/mL)、提取时间 20 min.在最佳提取工艺条件下,花色苷的平均得率达到 108.55 mg/100 g,RSD为 0.05%,与理论值(108.84 mg/100 g)的相对误差为0.27%.抗氧化活性试验研究表明,当紫薯花色苷和维生素C的浓度相同时,其各自的抗氧化活性的变化趋势一致,同时,紫薯花色苷对DPPH具有较好的清除能力且还原力也较好,紫薯花色苷的抗氧化能力强于维生素C.

Abstract

Using"Luozishu 4"as experimental material,the extraction process of purple sweet potato antho-cyanin was optimized by response surface methodology on the basis of single factor experiment,and the antioxi-dant activity of purple sweet potato anthocyanin was studied.The results showed that the order of the influence of three single factors on the yield of anthocyanin was C(HCl volume fraction)>B(material-liquid)>A(extraction time).The optimal extraction parameters of purple sweet potato anthocyanin were HCl volume fraction 0.75%,material-liquid ratio 1∶20(g/mL),and extraction time 20 min.Under the optimal extraction conditions,the aver-age yield of anthocyanin reached 108.55 mg/100 g,with RSD of 0.05%,and the relative error with the theoretical value(108.84 mg/100 g)was 0.27%.The antioxidant activity test showed that purple sweet potato anthocyanin had good scavenging ability to DPPH and good reducing ability.When the concentrations of purple sweet potato anthocyanin and vitamin C were the same,their antioxidant change trend were the same,and the antioxidant a-bility of purple sweet potato anthocyanin was stronger than that of vitamin C.

关键词

"漯紫薯4"/花色苷/响应面法/提取工艺/抗氧化活性

Key words

"Luozishu 4"/anthocyanins/response surface methodology/extraction process/antioxidant

引用本文复制引用

基金项目

现代农业产业技术体系(CARS-10-C-13)

河南省高等学校重点科研项目计划(24B550009)

漯河职业技术学院科技研发项目(2022-KJYF-03)

出版年

2024
粮食加工
陕西省粮油科学研究设计院

粮食加工

影响因子:0.341
ISSN:1007-6395
段落导航相关论文