Develop a novel tomato seed oil nano oral solution to broaden its processing pathway and enhance its added value.By combining high-speed shearing with ultrasonic treatment,tomato seed oil nanoparticles with small particle size,high encapsulation efficiency,and good uniformity were successfully prepared.Further research was conducted on the effects of ultrasound time,power,cooling method,and tomato seed oil addition on the characteristics of nanoparticles,and it was found that ultrasound treatment and rapid cooling can effectively reduce particle size and increase stability.In addition,the main components were analyzed by high-performance liquid chromatography,and the antioxidant and enzyme inhibitory activities of the nano oral solution were tested.The results showed that it had significant biological activity.This study provides a new approach for the efficient utilization of tomato seed oil and its application in the field of healthy food.