Screening Bax Interaction Proteins from 17.5 d Embryo Mouse cDNA Library
In order to study the formation of Bax protein complex which may play a role in apoptosis protein complex,we use the full length Bax as bait protein to screen 17.5 d embryo mouse cDNA library in GAL4 Yeast two hybrid system and a RACK1 protein was conferred. Interaction of Bax and RACK1 was validated in the eukaryotic expression vector with co-immunoprecipitation and immunofluorescence staining. Using 293T cells,overexpression lysates of Bax and RACK1 protein were harvested and co-immunoprecipitation experiment was conducted. The results showed that protein complex of Bax and RACK1 can be formed in vivo. After Transformation of Bax and RACK1 plasmid into 293T cells,tagging with different fluorescent antibodies,co-localization of Bax and RACK1 can be detected with a laser confocal microscope.
NETL
NSTL
万方数据
