Ascorbate acid plays important role in multiple physiological processes such as plant cell development and stress responses. Here, a cotton cDNA, enconding a 792 bp open reading frame and dehydroascorbate reductase3( GhDHAR3 ) protein of 263 amino acid residues, was cloned by RTPCR method from developing cotton fibers. Homology blast and phylogenetic tree analyses showed that GhDHAR3 contained conserved functional domain and belonged to the arabidopsis AtDHAR3 protein family. Motif search results indicated that GhDHAR3 protein contained typical GST N terminal family and GST C terminal subfamily motif belonging to the thioredoxin (TRX)-like superfamily and GST C-terminal alpha helical domain family respectively. Signal peptide was not found in Gh-DHAR3 protein sequence. Plant over-expression vector, constructed by enzyme digestion of Gh-DHAR3 and pCAMBIA2300 and successive ligation, was transformed of tobacco. Transgenic tobacco plants were obtained successfully after molecular identification.
CottonDehydroascorbate reductaseConstruction of plant expression vectorGenetictransformation
NETL
NSTL
万方数据
维普
