Chromosome Sectioning of Prunus mume Sieb.et Zucc All the Year Around
In order to pick out the best method of chromosome sectioning based on chromosome preparation of Prunus mume all the year around, five kinds of meristematic tissues, including the immature seeds, growing points by root tissue culture, growing points of one-year-old seedlings, stem tips of hydroponic scions and shoot buds after blooms, were obtained from cv. Fenban of Prunus mume Sieb. Et Zucc and served as materials. The wall degradation hypotonic flame-drying method was improved. The results showed that all tested materials were suitable for chromosome sectioning. The materials were fixed in acetic acid-alcohol under the temperature of -20℃ and then incubated in a pectolytic enzyme mixture at 37 ℃. After that some slides were passed through flame and air-dried. Some were dyed and pressed with cover glasses. Without pretreatment and hypotonic treatment, chromosomes stretch to a great extent. Besides, it was convenient for work and also improved the efficiency of chromosome sectioning.
NETL
NSTL
万方数据
维普
