Effects of Different Dietary Energy on IGF-1 and IGF-1R Gene Expressions in Skin Tissue of Weanling Rex Rabbits
The experiment was conducted to research the effects of different dietary energy on gene expressions of IGF-1 and IGF-1R in skin tissue of weaning rex rabbits.One hundred and sixty 45d aged rex rabbits with an average body mass of (1 133±71) g were randomly divided into 4 groups with 40 replicates,and each replicate contained 1 rabbit.Rabbits in the 4 groups were fed experimental diets containing 9.70 MJ/kg (Group Ⅰ),10.50 MJ/kg(GroupⅡ),11.30 MJ/kg(GroupⅢ),12.10 MJ/kg DE(GroupⅣ),respectively.The experiment included a pretrial period of 7 days and an experimental period of 60 days.At the age of 75 days and 105 days,1 cm2 skin tissue was collected from central back of slaughtered rabbits respectively and used for total RNA extraction and reverse transcription as well as gene expression of hair quality related IGF-1 and IGF-1R by real time PCR.The results showed that at the age of 75 days,the expressions ofIGF-1 gene was significantly higher in Ⅲ group than in Ⅰ group (P＜0.05) ; the expression of IGF-1R gene was significantly higher in Ⅲ group than in Ⅰ group (P＜0.05),too.At the age of 105 days,the expressions of both IGF-1 and IGF-1R gene were the highest in Ⅲ group,but the expressions of every group has no significant difference.The results indicated that the expressions of IGF-1 and IGF-1R gene were the highest when the digestive energy level was 11.30 MJ/kg during the growth period of rex rabbits.And skin hair follicles and wool could grow fast and steadily under the condition of sufficient energy supply.
Weanling rex rabbitEnergy levelSkin tissueGene expression
NETL
NSTL
万方数据
维普
