Identification and Analysis of RNA Editing Sites in the Chloroplast Transcripts of Ageratina adenophora
RNA editing is an important approach to regulate the gene expression at post-transcriptional levels in chloroplast transcript in terrestrial plants.This paper predicted and analyzed the RNA editing sites in the cp genome of A.adenophora using the Prep-Cp and CURE tools with molecular cloning and sequencing methods.Forty-two editing sites were identified and distributed in 19 proteincoding genes,of which all were C to U conversion.Among them,8 RNA editing sites occurred in the first position of codons,and the rest 34 sites occurred in the second position of codons.No RNA editing sites were detected in the third position of codon.Four genes and ten editing sites actual existed.Finally,the second structures and the transmembrane domains of these proteins were also analyzed using bioinformatics methods to identify the effect of the editing.Results indicated thatndhB-467 editing can cause an increase in transmembrane protein domains while editing inndhB-149 will change the secondary structure.
Ageratina adenophoraChloroplast genomeRNA editingIdentification and analysis
NETL
NSTL
万方数据
维普
