The aim of this study is to investigate the effects of Oxytropis glabra DC on content and transcriptional expression of α-mannosidase(AMA) in testis tissue,and revealed the toxicity mechanism of O.glabra DC.72 rats were divided into 4 groups randomly (the control group and experimental group Ⅰ,Ⅱ,Ⅲ),The dried plant of O.glabra DC was crushed,and different amounts of the grass powder(150 g/kg,300 g/kg and 450 g/kg,the swainsonine content was 30 mg/kg,60 mg/kg,90mg/kg respectively) were mixed with the feeds in the three experimental groups.The rats consumed the forages freely until typical symptoms were observed.2 rats were selected randomly from each group every week,the testicles were collected and the contents of AMA and its mRNA expression in different encephalic regions were measured.In 63d test period,We found that AMA in golgi and lysosomal expressed in testicles of all experimental groups,O,glabra DC inhibited the expression of testicles AMA1 and AMA2 mRNA,and the difference was obvious,the difference was not obvious in experimental group Ⅰ and control group (P＞ 0.05),the expression of AMA1 and AMA2 mRNA decreased in experimental group Ⅲ,and it was significantly lower than control group.The results showed that O.glabra DC had remarkable effects on contents and transcriptional expression of AMA in rat testis.
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