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甘蓝抗枯萎病基因FOC1植物表达载体构建及遗传转化

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为研究甘蓝枯萎病抗性基因FOC1的抗性功能,利用前期克隆的FOC1基因,以pBI121质粒为植物表达载体,采用同源重组法构建FOC1基因的过表达载体;将构建好的重组质粒采用冻融法转入根癌农杆菌LBA4404菌株中,并通过农杆菌介导的甘蓝外植体转化法对感病甘蓝进行遗传转化,利用载体特异引物对获得的转基因植株进行PCR鉴定.结果表明,最终成功构建FOC1基因的过表达载体pBI121-35S FOC1,并已成功整合到受体甘蓝基因组中.
Construction of Plant Expression Vector and Transformation of Fusarium Wilt Resistance GeneFOC1 to Brassica oleracea
In order to study the function of resistance gene of cabbage Fusarium wilt FOC1 in transgenic cabbage,the plant expression vector Pro35S::FOC1/pBI121 was constructed by homologous recombination and transformed into Agrobacterium tumefaciens strain LBA4404 by freeze-thawing method.The genetic transformation of B.oleracea was conducted by Agrobacterium-mediated plant transformation method.The independent transformants were detected by PCR method.The results showed that the pBI121-35S-FOC1 expression vector had successfully integrated into the genome of acceptor cabbage.

Brassica oleraceaFusarium wilt resistance genespBI121 vectorPlant expression vectorGenetic transformation

刘洁、马建、雷蕾、孙超、康俊根、颉建明

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甘肃农业大学园艺学院,兰州730070

北京市农林科学院蔬菜研究中心,农业部华北地区园艺作物生物学与种质创制重点实验室,北京 100097

甘蓝 抗枯萎病基因 pBI121质粒 载体构建 遗传转化

“十二五”国家科技支撑计划“十二五”国家科技支撑计划国家自然科学基金北京市科委项目

2012BAD02B012014BAD01B0831372065Z141105002314020

2017

10.7606/j.issn.1004-1389.2017.03.015

西北农业学报
西北农林科技大学,甘肃,宁夏,青海,新疆农（林）业科学院及青海,新疆畜牧（兽医）科学院及新疆农垦科学院

西北农业学报

CSTPCDCSCD北大核心
影响因子：0.629
ISSN：1004-1389
年,卷(期)：2017.26(3)
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