The aim of the present study was to identify and genetically analyze Paramphistomum sp.collected from Cervus nippon around Wenzhou region of Zhejiang province in China.Adult worms were identified based on morphological observation under microscopy after staining,and then the pcox1 and pnad1 genes were analyzed by PCR and sequencing.Morphological identification results showed that Paramphistomum sp.from C.nippon were close to P.cervi in external shape,size and internal organs.Comparative sequence analysis demonstrated that thepcox1 andpnad1 sequences from three C.nippon-derived Paramphistomum sp.were 446 bp and 505 bp in length and entirely consistent in similarity.Moreover,the homologies were the highest between isolates from C.nippon and P.cervi (KT198987) in GenBank,reached 98.2％ and 97.7％,respectively.Phylogenetic analysis revealed that the three Paramphistomum samples from C.nippon and the P.cervi (KT198987) locate in the same branch.These results indicated that the Paramphistomum sp.has high consistency with P.cervi in morphology and phylogenetic analysis,suggesting the Paramphistomum sp.sampled from the C.nippon in Wenzhou area is P.cervi.
NETL
NSTL
万方数据
维普
